|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
05/11/2021 |
Actualizado : |
05/11/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
REZENDE, S.; BANCHERO, S.; MIGUES, I.; CESIO, M.V.; RIVAS, F.; HEINZEN, H.; BESIL, M.N. |
Afiliación : |
SOFÍA REZENDE, University of the Republic of Uruguay, Chemistry Department of Litoral, Paysandú, Uruguay; Technological University, Southwest Regional Technological Institute, Paysandú, Uruguay.; SABRINA BANCHERO, Technological University, Southwest Regional Technological Institute, Paysandú, Uruguay.; IGNACIO MIGUES, University of the Republic of Uruguay, Faculty of Chemistry, Montevideo, Uruguay.; MARÍA VERONICA CESIO, University of the Republic of Uruguay, Faculty of Chemistry, Montevideo, Uruguay.; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HORACIO HEINZEN, University of the Republic of Uruguay, Faculty of Chemistry, Montevideo, Uruguay.; MARÍA NATALIA BESIL, University of the Republic of Uruguay, Chemistry Department of Litoral, Paysandú, Uruguay. |
Título : |
Variability levels of selected amino acids among mandarins produced in Uruguay. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Ecletica Quimica, 2021, Volume 46, Issue 4, Pages 47-59. Gold Open Access. doi: https://doi.org/10.26850/1678-4618eqj.v46.4.2021.p47-59 |
ISSN : |
0100-4670 (print); 1678-4618 (electronic) |
DOI : |
10.26850/1678-4618eqj.v46.4.201.p47-59 |
Idioma : |
Inglés |
Notas : |
Article history: Received: July 27, 2020; Accepted: July 16, 2021; Published: October 01, 2021.
Section Editor: Assis Vicente Benedetti.
Corresponding author: María Natalia Besil. Email address: nbesil@fq.edu.uy |
Contenido : |
ABSTRACT.- Nutraceutical properties of mandarins are of great interest to promote their consumption. The occurrence of free amino acids in foods is relevant to assess the nutritional value of it. To learn more about the amino acids' occurrence and variability between species, a targeted metabolomics study in 'Ellendale', 'Willowleaf' and 'Page' varieties was performed through ion exchange liquid chromatography coupled to tandem mass spectrometry. The studied amino acids were asparagine, glutamic acid, glutamine, histidine, methionine, phenylalanine, proline, threonine and tyrosine. The difference between two consecutive seasons was evaluated, as well as the influence of fruit maturity of 'Page' collected in two periods of 2015. The analytical methodology was validated. The concentration of the compounds through principal component analysis, separated well apart the three cultivars in both harvests, showing a particular profile for each of them. When comparing mature and immature cultivar 'Page', the amino acids with higher levels in mature samples were histidine, asparagine, glutamine and glutamic acid. The profiles were different due to genetic diversity, and the climatic conditions. These results add value to citric production. © 2021 Atlantis Livros Ltda.. All rights reserved. |
Palabras claves : |
Amino acids; High performance liquid chromatography-mass spectrometry; Mandarins; Nutraceuticals. |
Thesagro : |
CITRUS. |
Asunto categoría : |
F01 Cultivo |
URL : |
https://revista.iq.unesp.br/ojs/index.php/ecletica/article/download/1206/1208
https://revista.iq.unesp.br/ojs/index.php/ecletica/article/view/1206
|
Marc : |
LEADER 02454naa a2200289 a 4500 001 1062516 005 2021-11-05 008 2021 bl uuuu u00u1 u #d 022 $a0100-4670 (print); 1678-4618 (electronic) 024 7 $a10.26850/1678-4618eqj.v46.4.201.p47-59$2DOI 100 1 $aREZENDE, S. 245 $aVariability levels of selected amino acids among mandarins produced in Uruguay.$h[electronic resource] 260 $c2021 500 $aArticle history: Received: July 27, 2020; Accepted: July 16, 2021; Published: October 01, 2021. Section Editor: Assis Vicente Benedetti. Corresponding author: María Natalia Besil. Email address: nbesil@fq.edu.uy 520 $aABSTRACT.- Nutraceutical properties of mandarins are of great interest to promote their consumption. The occurrence of free amino acids in foods is relevant to assess the nutritional value of it. To learn more about the amino acids' occurrence and variability between species, a targeted metabolomics study in 'Ellendale', 'Willowleaf' and 'Page' varieties was performed through ion exchange liquid chromatography coupled to tandem mass spectrometry. The studied amino acids were asparagine, glutamic acid, glutamine, histidine, methionine, phenylalanine, proline, threonine and tyrosine. The difference between two consecutive seasons was evaluated, as well as the influence of fruit maturity of 'Page' collected in two periods of 2015. The analytical methodology was validated. The concentration of the compounds through principal component analysis, separated well apart the three cultivars in both harvests, showing a particular profile for each of them. When comparing mature and immature cultivar 'Page', the amino acids with higher levels in mature samples were histidine, asparagine, glutamine and glutamic acid. The profiles were different due to genetic diversity, and the climatic conditions. These results add value to citric production. © 2021 Atlantis Livros Ltda.. All rights reserved. 650 $aCITRUS 653 $aAmino acids 653 $aHigh performance liquid chromatography-mass spectrometry 653 $aMandarins 653 $aNutraceuticals 700 1 $aBANCHERO, S. 700 1 $aMIGUES, I. 700 1 $aCESIO, M.V. 700 1 $aRIVAS, F. 700 1 $aHEINZEN, H. 700 1 $aBESIL, M.N. 773 $tEcletica Quimica, 2021, Volume 46, Issue 4, Pages 47-59. Gold Open Access. doi: https://doi.org/10.26850/1678-4618eqj.v46.4.2021.p47-59
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
13/06/2023 |
Actualizado : |
01/09/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
GIANNITTI, F.; DORSCH, M.; FERNÁNDEZ-CIGANDA, S.; RABAZA, A.; VÁZQUEZ, S.; CÉSAR, C.; HURTADO, J.; GREIF, G.; RABENECK, D.; BHATNAGAR, J.; RITTER, J. |
Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATÍAS ANDRÉS DORSCH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA VIRGINIA RABAZA MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SEBASTIÁN VÁZQUEZ, Private practice, Colonia del Sacramento, Uruguay; DEBORAH CÉSAR; JOAQUÍN HURTADO, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay; GONZALO GREIF, Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay; DEMI B. RABENECK, Infectious Diseases Pathology Branch (IDPB), Centers for Disease Control and Prevention (CDC), Atlanta, GA; JULU BHATNAGAR, Infectious Diseases Pathology Branch (IDPB), Centers for Disease Control and Prevention (CDC), Atlanta, GA; JANA M. RITTER, Infectious Diseases Pathology Branch (IDPB), Centers for Disease Control and Prevention (CDC), Atlanta, GA. |
Título : |
Canine leproid granuloma caused by a member of the Mycobacterium tuberculosis complex. (Brief Report). |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Journal of Veterinary Diagnostic Investigation. 2023, Volume 35, Issue 4, pages 439-443. https://doi.org/10.1177/10406387231176816 |
ISSN : |
1943-4936 (online). |
DOI : |
10.1177/10406387231176816 |
Idioma : |
Inglés |
Notas : |
Article history: First published online May 19, 2023; Issue published July 2023. -- Corresponding author: Federico Giannitti, Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50, km 11 (70006), La Estanzuela, Colonia, Uruguay. fgiannitti@inia.org.uy -- Funding: Funded by research grant PL_27 N-23398 from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Contenido : |
ABSTRACT.- Canine leproid granuloma (CLG) is a chronic form of dermatitis that has been associated with nontuberculous mycobacterial infections in Africa, Oceania, the Americas, and Europe. We report here a case of CLG associated with a member of the Mycobacterium tuberculosis complex (MTBC), which could be of public health concern. An 8-y-old pet dog developed 0.5-1-cm diameter, raised, firm, nonpruritic, alopecic, painless skin nodules on the external aspects of both pinnae. Histologic examination revealed severe pyogranulomatous dermatitis with intracellular Ziehl-Neelsen-positive bacilli that were immunoreactive by immunohistochemistry using a polyclonal primary antibody that recognizes tuberculous and nontuberculous Mycobacterium species. DNA extracted from formalin-fixed, paraffin-embedded skin sections was tested by a Mycobacterium genus-specific nested PCR assay targeting the 16S rRNA gene. BLAST sequence analysis of 214-bp and 178-bp amplicons showed 99.5% identity with members of the MTBC; however, the agent could not be identified at the species level. Although CLG has been associated traditionally with nontuberculous mycobacterial infections, the role of Mycobacterium spp. within the MTBC as a cause of this condition, and the role of dogs with CLG as possible sources of MTBC to other animals and humans, should not be disregarded given its zoonotic potential. © 2023 The Author(s). |
Palabras claves : |
Canine leproid granuloma; Dermatitis; Dogs; Mycobacterium tuberculosis complex; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Pyogranuloma. |
Asunto categoría : |
L40 Estructura animal |
Marc : |
LEADER 02982naa a2200361 a 4500 001 1064188 005 2023-09-01 008 2023 bl uuuu u00u1 u #d 022 $a1943-4936 (online). 024 7 $a10.1177/10406387231176816$2DOI 100 1 $aGIANNITTI, F. 245 $aCanine leproid granuloma caused by a member of the Mycobacterium tuberculosis complex. (Brief Report).$h[electronic resource] 260 $c2023 500 $aArticle history: First published online May 19, 2023; Issue published July 2023. -- Corresponding author: Federico Giannitti, Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Ruta 50, km 11 (70006), La Estanzuela, Colonia, Uruguay. fgiannitti@inia.org.uy -- Funding: Funded by research grant PL_27 N-23398 from the Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. 520 $aABSTRACT.- Canine leproid granuloma (CLG) is a chronic form of dermatitis that has been associated with nontuberculous mycobacterial infections in Africa, Oceania, the Americas, and Europe. We report here a case of CLG associated with a member of the Mycobacterium tuberculosis complex (MTBC), which could be of public health concern. An 8-y-old pet dog developed 0.5-1-cm diameter, raised, firm, nonpruritic, alopecic, painless skin nodules on the external aspects of both pinnae. Histologic examination revealed severe pyogranulomatous dermatitis with intracellular Ziehl-Neelsen-positive bacilli that were immunoreactive by immunohistochemistry using a polyclonal primary antibody that recognizes tuberculous and nontuberculous Mycobacterium species. DNA extracted from formalin-fixed, paraffin-embedded skin sections was tested by a Mycobacterium genus-specific nested PCR assay targeting the 16S rRNA gene. BLAST sequence analysis of 214-bp and 178-bp amplicons showed 99.5% identity with members of the MTBC; however, the agent could not be identified at the species level. Although CLG has been associated traditionally with nontuberculous mycobacterial infections, the role of Mycobacterium spp. within the MTBC as a cause of this condition, and the role of dogs with CLG as possible sources of MTBC to other animals and humans, should not be disregarded given its zoonotic potential. © 2023 The Author(s). 653 $aCanine leproid granuloma 653 $aDermatitis 653 $aDogs 653 $aMycobacterium tuberculosis complex 653 $aPathology 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aPyogranuloma 700 1 $aDORSCH, M. 700 1 $aFERNÁNDEZ-CIGANDA, S. 700 1 $aRABAZA, A. 700 1 $aVÁZQUEZ, S. 700 1 $aCÉSAR, C. 700 1 $aHURTADO, J. 700 1 $aGREIF, G. 700 1 $aRABENECK, D. 700 1 $aBHATNAGAR, J. 700 1 $aRITTER, J. 773 $tJournal of Veterinary Diagnostic Investigation. 2023, Volume 35, Issue 4, pages 439-443. https://doi.org/10.1177/10406387231176816
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|